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Changes of transplantation pecurialities of fetal liver hemopoietic stem cells after short-term culture in diffusion chamber in vivo

Wei Qisheng, Wu Zuze

Institute of Radiation Medicine, Academy of Military Medical Science. Beijing, China

Abstract

Fetal liver cells from LACA inbred mice after 6 days of in vivo culture were
transplanted into a group of syngeneic irradiated adult mice, the seeding efficiency of hemopoictic stem cells both in recipient spleen and bone imarrow, in comparing;with the normal fetal liver cells, was obviously increased. This effect was completely abolished in allogencic transplantation of fetal liver cells i:rom LACA mice into.lethally irradiated C6, adult mice. Experimental results showed that ontogenetic and histoincompatibal barriers are the major factors determining the success of allogeneic fetal liver transplantation. In vitro or in vivo culture of fetal liver cells may create favourable conditions to accelerate the development of hemopoietic stem cells from the fetal immature stage to adult mature state, and therefore makes them adaptable to lodge in adult bone marrow microenvironment. The shortterm in vivo culture as reported in this paper showed an effect of transformation of some genetic characters across the ontogenetic barrier but could not overcome the barrier of allogenetic resistance. Hence it is likely that the histoincompatable barrier plays more important role than ontogenetic barrier to ensure the successful engraftment in allogenetic fetal liver transplantation and the final achievement
to this end requires further removal of this obstacle.

Key words: Fetal liver;Hemopoiesis;Diffusion chamber;Transplantation;Stem cells

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Citing This Article：

Wei Qisheng, Wu Zuze. Changes of transplantation pecurialities of fetal liver hemopoietic stem cells after short-term culture in diffusion chamber in vivo. Acta Physiol Sin 1987; 39 (2): (in Chinese with English abstract).