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Effects of niflumic acid on the proliferation of human hepatoma cells

TI AN Jing, TAO Ling, CAO Yuir Xin, DONG Ling, HU YirZhen, YANG An Gang, Zhou Shisheng

Department of Physiology,lmmunology, and Biochemistry and Molecular Biology, the Fourth Military Medical University, Xi'an 710032

Abstract

The purpose of this work was to investigate the effects of niflumic acid(NF A)，a chloride channel Mocker，on the proliferation of human hepatoma cell line(H H CC).Cell proliferation was analyzed by cell count and MTT assay .Cell cycle analysis was carried out by flow cytometry.[Ca~(2+)]，was determined by laser scanning confocal system.It was found that NFA decreased significantly the cell number and the MTT optical density(OD) of HHCC cells，and that the OD value was reversed after washout of NFA.Compared with control，NFA blocked cell cycle progression in G, phase.Extracellular application of NFA(100 p mol/ L) induced a rapid decrease in[Ca~(2+)]，.These findings demonstrate that
blockage of chloride channels by NFA induces growth arrest of H HCC in G, phase，which may be due to the inhibition of Ca~(2+)/Ca 1VF dependent signaling pathways.

Key words: hepatoma;Proliferation;chloride channel;Ca~(2+)

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Citing This Article：

TI AN Jing, TAO Ling, CAO Yuir Xin, DONG Ling, HU YirZhen, YANG An Gang, Zhou Shisheng. Effects of niflumic acid on the proliferation of human hepatoma cells. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).