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Effect of protein kinase C on Kv channel in rat bronchial smooth muscle

LI U Maxr Sheng, XU Yong Jian, ZHANG Zheir Xiang, NI Wang, CHEN Shi-Xin

Department of Respiratory Medicine，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030

Abstract

The effect of protein kinase C(P KC) signaling pathway on the activity of voltage- dependent delayed rectifier potassium channel(Kv) and the expression of Kv isoform Kvl·5 in rat bronchial smooth cells(BS MCs) were investigated with whole-cell patch clamp，Western blot and RT-PCR techniques .The results showed:(1)phorbol 12- myristate 13-acetate(P MA)，a PKC activator，caused a
significant inhibition of Kv channel currents in rat BS MCs .The inhibition was partly abolished by Ro318220，a PKC inhibitor.(2) P MA caused a significant suppression of the expression of Kvl·5 m RNA and protein in rat BS MCs .These effects were attenuated by Ro31一8220 .The results suggest that in rat BS MCs P KC activation inhibits Kv currents and down regulates the expression of Kvl 5.

Key words: bronchus;Smooth muscle cells;voltage-dependent delayed rectifier potassium channel;protein kinase C

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Citing This Article：

LI U Maxr Sheng, XU Yong Jian, ZHANG Zheir Xiang, NI Wang, CHEN Shi-Xin. Effect of protein kinase C on Kv channel in rat bronchial smooth muscle. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).