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EFFECTS OF GONADAL STEROID HORMONES ON HYPOTHALAMIC VASOPRESSIN mRNA LEVEL IN MALE AND FEMALE RATS

DAI WEN-JIE, LU Li-Min, YAo TAI

Department of Physiology, shanyhai Medical University, Shanghai 200032)

Abstract

Experiments were carried out in 10-11一week old gonadectomized male and fe-
male Sprague-Dawley rats. Dot-blot analysis and 31一end digoxigenin-labeled 26 meroligonucleotide probe was used in detecting the mRNA level hypothalamic vasopressin (AVP).The basal hypothalamic AVP-mRNA level in the sham-operated intact males was 4 5%higher than that in the sham-operated intact females (P<0. 05).Plasma osmolality was also higher in the sham-operated intact males than in the sham-operated intact females (P<0. 05).The hypothalamic AVP-mRNA level in ovariectomized rats was 30%higher than that in sham-operated intact females (P< 0. 05).Although the hypothalamic AVP mRNA level tended to be lower in castrated males than in sham-opcrated intact males，the difference was not statistically significant. The difference in plasma osmolality between gonadectomized males and females was statistically insignifcant. In castrated males，hypothalamic AVP-mRNA level was decreased following sc injection of estradiol(P<0.05)，but testosterone，progesterone or a combination of
estradiol and progesterone were without effect.

Key words: Vasopressin;;mRNA;

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Citing This Article：

DAI WEN-JIE, LU Li-Min, YAo TAI. EFFECTS OF GONADAL STEROID HORMONES ON HYPOTHALAMIC VASOPRESSIN mRNA LEVEL IN MALE AND FEMALE RATS. Acta Physiol Sin 1996; 48 (6): (in Chinese with English abstract).