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ANGIOTENSIN Ⅱ RELEASE AND ANTI-ELECTROACUPUNCTURE ANALGESIA IN SPINAL CORD

SHEN SHANG, LI JUN, WANG XIAO-MIN AND HAN JI-SHENG

(Neuroscience Research Center, Beijing Medical University, Beijing 100083)

Abstract

Changes in the content of angiotensin Ⅱ( (A Ⅱ)immunoreactivity (it)in rat
spinal perfusate induced by electroacupuncture (EA) stimulation of different frequencies were measuerd by radioimmunoassay (RIA).The results were analyzed in relation to the role of opioid recepor.(1)2 Hz EA produced a 20%(P>0. 05) decrease in A Ⅱ -it content in the spinal perfusate o 15 Hz EA produced an even more decrease (62%，P<0. O l)，whereas 100 Hz produced a significant increase(65%，P<0. 05).(2) The release in spinal A Ⅱ一it produced by 15 Hz EA was reversed by the opioid antagonist naloxone to a of 125%highter than that of the control (PLO. 05)，suggesting that 1.5 Hz EA may accelerate the release of endogenous opioids to suppress the release of A Ⅱ.(3) This was substantiated by the finding that intrathecal (i. t.)injection of the selective mu agonist ohmefentanyl produced a dramatic suppression(200a，
P< 0. 05) of A Ⅱ release，but not by delta and kappa agonist. (4)Intrathecal injection of salarasin，the angiotensin receptor antagonist，produced a significant potentiation of the analgesia produced by 100 Hz EA，but not that produced by 2 or 15 Hz EA. It is concluded that 15 Hz EA may induce the release of endogenous opioids acting on p opioid receptor so as to suppress A If release，and that 100 Hz EA may acceleate the release of A Ⅱ servinp- as a brake for 100 Hz EA-induced analpensia.

Key words: angiotensin Ⅱ;electroacupuncture analgesia;frequency;immunoreactivity;Opioid receptor;spinal cord

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Citing This Article：

SHEN SHANG, LI JUN, WANG XIAO-MIN AND HAN JI-SHENG. ANGIOTENSIN Ⅱ RELEASE AND ANTI-ELECTROACUPUNCTURE ANALGESIA IN SPINAL CORD . Acta Physiol Sin 1996; 48 (6): (in Chinese with English abstract).