Issue Archive

THE TRANSDUCING PATHWAY OF Ca~(2+) INFLUX DURING PROGESTERONE-INITIATED ACROSOME REACTION OF GUINEA PIG SPERM

JIA ZHEN-YU, YUAN YU-YING, SHI QI-XIAN

(Zhejiaug Academy of Medical Sciences, HangzJau 310013)

Abstract

Progesterone and #gamma#-aminobutyric acid (GABA) could initiate the acrosome reaction (AR) in guinea pig spermatozoa，which was independent of extracellular Cl一in vdro，and a potentiation effect between progesterone and GABA was demonstrated. Antagonists of GABAA receptor/Cl一channel, picrotoxin and bicuculline，have no effect on the progesterone-induced AR，but they can significantly inhibit the GABA-induced AR in Cl--containing medium. While in Cl--deficient medium，picrotoxin has significant inhibitory effect on both progesterone- and GABA-induced AR，both progesterone- and GABA-induced AR are inhibited by the Call channel blocker，nifedipine. These results suggest that a GABAA/Cl一receptor complex and a Call channel might be involved in
the influx of extracellular Call during the progesterone-induced AR.

Key words: progesterone;#gamma#-aminobutyric acid (GABA);acrosome reaction (AR);GABAA-like receptor;;

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

JIA ZHEN-YU, YUAN YU-YING, SHI QI-XIAN. THE TRANSDUCING PATHWAY OF Ca~(2+) INFLUX DURING PROGESTERONE-INITIATED ACROSOME REACTION OF GUINEA PIG SPERM. Acta Physiol Sin 1997; 49 (2): (in Chinese with English abstract).