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THE ROLES OF NITRIC OXIDE AND PROSTAGLANDINS IN THE ENDOTOXIN-INDUCED RELEAS RELATED PEPTIDE (CGRP) IN RAT MESENTERIC ARTERYE OF CALCITONIN GENE-

WANG XIAN, WU ZHONG-XIN, TANG YUE-MING , HAN QI-DE

(Institute of Vascular Medicine, The Third Hospital, Beijing Medical University, Beijing 100083)

Abstract

The mechanism of endotoxin (ETX)-induced release of CGRP was studied in isolated mesenteric arterial bed. ETX (50 }tg/ml) caused a 16-fold increase of the release of CGRP. The effect of ETX was enhanced by L-arginine (L-Arg)，a substratum of nitric oxide synthase (NOS)，by 41%，but inhibited by NG- nitro- L- arginine (LNNA)，an selective inhibitor of NOS，L-NNA，and methylium blue (MB)，an inhibitor of guanylate cyclase，respectively by 35%and 36%.These observations suggested that the effect of ETX is，at least partially，mediated by elevation of intracellular cGMP induced by NO. When endothelial cells were removed，the above responses of L-NNA and L-Arg still existed，indicating that ETX activated neuronal，rather than endothelial，NOS. Indomethacin and ibupofen，inhibitors of cyclooxygenase，also inhibited ETX-induced CGRP release by 34写and 39%respectively. When L-NNA and indomethacin were both incubated，no additive effects were discernible. The data suggest that ETX triggers CGRP release partially through activation of NOS located in
perivascular sensory nerve and increase of NO，which is followed by activation of c}clooxygenase.

Key words: Calcitonin gene-related peptide;Endotoxin;nitro oxide;cyclooxygenase

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Citing This Article：

WANG XIAN, WU ZHONG-XIN, TANG YUE-MING , HAN QI-DE. THE ROLES OF NITRIC OXIDE AND PROSTAGLANDINS IN THE ENDOTOXIN-INDUCED RELEAS RELATED PEPTIDE (CGRP) IN RAT MESENTERIC ARTERYE OF CALCITONIN GENE- . Acta Physiol Sin 1996; 48 (3): (in Chinese with English abstract).