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THE ROLES OF PROTEIN KINASE C AND Na+一H+ EXCHANGE IN THE ANGIOTENSIN II-INDUCED HYPERTROPHIC RESPONSE

DING XIAO-LING, ZHOU CHENG-YU , LI YUN-XIA

(Laboratory of Cardiarmculam Physidogy, Huam Medical University, Changsha 410078)

Abstract

In order to study the roles of protein kinase C (PKC) and Na+一H+ exchange in [Sar~(1)] ANG ff -induced hypertrophic response，3H-Leucine incorporation into serumfree cultured neonatal rat cardiorts,yocytes were interfered with staurosporine，phorbol 12-myristate 13-acetate (PMA) or amiloride. With [Sar~(1)] ANG I，a dose dependent augumentation of protein synthesis was observed. Activator of PKC (PMA) could also accelerate the rate of protein synthesis to a significant extent，while pretreatment of cardiac myocytes with PKC inhibitor (staurosporine)or Na+一H+ exchanger inhibitor (amiloride) would block [Sar~(1) ] ANG I -induced increase 3H-Leucine incorporation. These results demonstrated that activation of PKC and Na+-H+ exchange might mediate
the hypertrophic response initiated by [Sar~(1)] ANG if . However，inhibition of cellular Na+-H+ exchange did not affect the increase in protein synthesis due to activation of PKC，a finding indicating that the two resorts to regulate cell growth are mediated by independent pathways.

Key words: Protein synthesis; Angiotensin 1l;Protein kinase C;Na+-H+ exchange;cultured cardiac myocytes; neonatal rat

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Citing This Article：

DING XIAO-LING, ZHOU CHENG-YU , LI YUN-XIA. THE ROLES OF PROTEIN KINASE C AND Na+一H+ EXCHANGE IN THE ANGIOTENSIN II-INDUCED HYPERTROPHIC RESPONSE. Acta Physiol Sin 1994; 46 (5): (in Chinese with English abstract).