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Effects of hypoxic exposure on coordinative expression of cytochrome oxidase subunits Ⅰ and Ⅳ in rat cerebral cortex

Tan Xiaoling, Liu Junze, Cao Lifei, Deng Zhongcai, Li Yinghe

Department of Pathophysiology,College of High Altitude Military Medicine,the Third Military Medical University.Chongqing 400038

Abstract

The results showed that COX I mRNA increased significantly at 2 and 5 d, and decreased to the control level at 15 and 30 d; COX IV mRNA remarkably increased at 2, 5 and 15 d, and dropped below the control level at 30 d. The mRNA ratio of COX IV to COX I reached a peak at 15 d, but showed no differences between other time points. The Western blot analysis of COX I and COX IV in isolated at cerebral cortex mitochondria showed no obvious changes during hypoxic exposure. Our findings demonstrate that hypoxia can affect mRNA expression of COX I and COX IV and their coordination, while protein expression of both subunits are stable and coordinative. This study suggests that the expression of COX I and COX IV proteins during hypoxic exposure is coordinately regulated by post-transcriptional mechanisms.

Key words: Cytochrome oxidase;Gene expression;Cerebral cortex;Rat;Hypoxia

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Citing This Article：

Tan Xiaoling, Liu Junze, Cao Lifei, Deng Zhongcai, Li Yinghe. Effects of hypoxic exposure on coordinative expression of cytochrome oxidase subunits Ⅰ and Ⅳ in rat cerebral cortex. Acta Physiol Sin 2002; 54 (6): (in Chinese with English abstract).