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Effect of a proteinase-activated receptor-2 (PAR-2) agonist on tryptase release from human mast cells

He Shaoheng, Xie Hua, He Yongsong

Allergy and Inflammation Research Institute,the Medical College of Shantou University.Shantou 515031,Guangdong；China

Abstract

Proteinase-activated receptor-2 (PAR-2) expression has been observed on numerous cell types. However, little is known about the functional expression of PAR-2 in human mast cells. In the current study, the actions of a PAR-2 agonist trans-cinnamoyl-Leu-Ile-Gly-Arg-Leu-Orn-amide (tc-LIGRLO) on tryptase release from dispersed human colonic mast cells were examined. The results showed that tc-LIGRLO was aboe to induce a fold increase in tryptase release over the basal level following a 15 min incubation of colonic mast cells, whereas tc-OLRGIL did not have any effect on tryptase release. The potency of tc-LIGRLO appeared greater than that of anti-IgE and calcium ionophore A23187 (CI) in induction of tryptase release. Extending the incubation time to 30 min had no significant effect on the actions of tc-LIGRLO or anti-IgE. In the time course study, it was observed that the tryptase release from mast cells induced by tc-LIGRLO started at 1 min and peaked at 3 min following incubation. The above-mentioned results indicate that tc-LIGRLO is a potent stimulus of tryptase release from human mast cells, which strongly suggests that PAR-2s are expressed in human mast cells.

Key words: Proteinase activated receptor-2;Tryptase;Mast cell

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Citing This Article：

He Shaoheng, Xie Hua, He Yongsong. Effect of a proteinase-activated receptor-2 (PAR-2) agonist on tryptase release from human mast cells. Acta Physiol Sin 2002; 54 (6): (in Chinese with English abstract).