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Action of genistein on tension of isolated rabbit femoral artery and its mechanism

Ji Ensheng, Li Qing, He Ruirong

Department of Physiology,Institute of Basic Medicine,Hebei Medical University.Shijiazhuang 050017,Hebei

Abstract

The results are as follows: (1) genistein (10-40 #mu#mol/L) relaxed femoral arterial rings in a concentration-dependent manner under the condition of precontraction induced by phenylephrine (PE, 1#mu#mol/L); (2) removal of the endothelium significantly inhibited genistein-induced relaxation; (3) pretreatment with NOS inhibitor N~(G)-nitro-L-arginine methyl ester (L-NAME, 100 #mu#mol/L) also significantly inhibited this relaxation by genistein, implying that the concentration-dependent vasorelaxation caused by genistein is endothelium-dependent and involved nitric oxide; and (4) pretreatment with an L-type calcium channel agonist, Bay K 8644 (0.5 #mu#mol/L), also significantly inhibited the genistein-induced relaxation in both endothelium-intact and endothelium-denuded rings. The results suggest that the genistein-induced vascular relaxation of these rabbit arteries is partially endothelium-dependent and involves calcium antagonistic mechanism.

Key words: Genistein;Nitric oxide;Endothelial function;Bay K 8644

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Citing This Article：

Ji Ensheng, Li Qing, He Ruirong. Action of genistein on tension of isolated rabbit femoral artery and its mechanism. Acta Physiol Sin 2002; 54 (5): (in Chinese with English abstract).