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Isoproterenol-induced activation of MAPK, NF#kappa#B and JAK/STAT pathway in mouse myocardium

Yin Feng, Zhu Yun, Li Ping, Han Qide, Zhang Youyi

Institute of Vascular Medicine,Peking University Third Hospital,The Reference Laboratory of Education Ministry on Molecular Cardiology.Beijing 100083

Abstract

This study was aimed to determine the in vivo signal transduction pathway responsible for isoproterenol (ISO)-induced cardiac hypertrophy or remodeling. Mice were treated with ISO (15 mg/kg body weight) or vehicle by intraperitoneal injection (i.p.). Activation of mitogen-activted protein kinase (MAPK), NF-#kappa#B and JAK/STAT pathway in the left ventricular myocardium was measured by Western blot analysis. ISO significantly activated MAPK (ERK1/2 and p38) at early phase (5 min); biphasic activation of NF-#kappa#B was observed in our in vivo study; and ISO caused a delayed STAT3 activation (at 60 to 240 min) in mouse myocardium. Taken together, these results indicate that ISO activates these signal transduction pathways in different time course.

Key words: Isoproterenol;Myocardium;Mitogen-activted;Protein kinase （MAPK）;NFKB JAK/STAT

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Citing This Article：

Yin Feng, Zhu Yun, Li Ping, Han Qide, Zhang Youyi. Isoproterenol-induced activation of MAPK, NF#kappa#B and JAK/STAT pathway in mouse myocardium. Acta Physiol Sin 2003; 55 (4): (in Chinese with English abstract).