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Production of somatic hybrid plants between sour orange and sweet orange via electrofusion for creation of CTV-resistant rootstock

Liu Jihong, Deng Xiuxin

National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University.Wuhan 430070,Hubei

Abstract

Leaf-derived protoplasts of sour orange (Citrus aurantium L.) were electrically fused with embryogenic protoplasts of sweet orange (C. sinensis Osbeck cv. Shamouti) based on fusion parameters as previously reported. Forty plants have been obtained from fusion products. Chromosome counting showed that the plants were tetraploids with 36 chromosomes. The plants have thick petiole-winged leaves, indicative of the characteristics of polyploid. Two 10-bp random primers were utilized to identify the hybridity of the plants. Bands specific to fusion parents were detected in the amplification band patterns of the regenerated hybrid plants for the primers. Chromosome counting and RAPD analysis demonstrated that interspecific allotetraploid somatic hybrid plants have been successfully recovered between sour organge and sweet orange. The regeneration of the somatic hybrid plants between sour orange and sweet orange provides good experimental materials for selecting novel rootstock possessing superior traits of sour orange coupled with CTV resistance.

Key words: Sour orange;Sweet orange;Electrofusion;Tetraploid;Somatic hybrid;Citrus tristeza virus

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Citing This Article：

Liu Jihong, Deng Xiuxin. Production of somatic hybrid plants between sour orange and sweet orange via electrofusion for creation of CTV-resistant rootstock. Acta Physiol Sin 2001; 53 (6): (in Chinese with English abstract).