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Effect of protein kinase C on inhibition of nitric oxide synthesis in cultured neonatal rat cardiomyocytes by angiotensin II

Fu Shigan, Liu Peiqing, Lu Wei, Gong Suzhen, Pan Jingyun

Department of Physiology,Sun Yat-Sen University of Medical Sciences.Guangzhou 510080,Guangdong

Abstract

NO production was decreased by Ang II in a dose-dependent manner but increased by L-Arg. The Saralasin, an antagonist of AngII receptor, inhibited the effect of AngII on NO production. The effect of AngII on NO production was inhibited by NOS blocker N~(G)-nitro-L-arginine methyl ester (L-NAME) but not by L-Arg. Pretreatment of Phorbol 12-myristate 13-acetate (PMA), a PKC activator, decreased NO concentration significantly. This effect was strengthened by L-NAME. Staurosporine, a PKC inhibitor, abolished the inhibiting effect of AngII on production of NO.

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Citing This Article：

Fu Shigan, Liu Peiqing, Lu Wei, Gong Suzhen, Pan Jingyun. Effect of protein kinase C on inhibition of nitric oxide synthesis in cultured neonatal rat cardiomyocytes by angiotensin II. Acta Physiol Sin 2000; 52 (4): (in Chinese with English abstract).