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Effect of active oxygen on activity of phosphoenolpyruvate carboxylase from Amaranthus tricolor

Lin Zhifang, Peng Changlian, Lin Guizhu

South China Institute of Botany,Chinese Academy of Sciences.Guangzhou 510650,Guangdong

Abstract

Phosphoenolpyruvate carboxylase (PEPC) was partially purified from leaves of Amaranthus tricolor, then treated with active oxygen species such as H_(2)O_(2), O~(.)_(2) and OH· for 15 min. Under oxidative condition, PEPC activity was inhibited by 10%-17% depending on the species and concentration of active oxygen. DMSO, a hydroxyl radical scavenger, stimulated the PEPC activity by 29%-48%, and altered the spectra of ultraviolet bsorption and differential absorption spectra. These results imply that oxidative stress leads to the modification of structure and conformation of PEPC and a decrease in activity.

Key words: Active oxygen;Activity of phosphoenolpyruvate carboxylase;Amaranthus tricolor;Ultraviolet difference spectra

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Citing This Article：

Lin Zhifang, Peng Changlian, Lin Guizhu. Effect of active oxygen on activity of phosphoenolpyruvate carboxylase from Amaranthus tricolor. Acta Physiol Sin 2000; 52 (1): (in Chinese with English abstract).