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Inhibitory effects of antisense oligonucleotides targeting mitogen-activated protein kinase (MAPK) mRNA on neonatal rat cardiac fibroblast proliferation induced by AngII and EGF

Ding Bo, Huang Shaoling, Li Yunxia

Laboratory of Cardiovascular Physiology,Hunan Medical University.Changsha 410078,Hunan

Abstract

In the present study, the antisense oligodeoxynucleotide (ODN) approach was used to investigate whether mitogen-activated protein kinase (MAPK) is necessary for the proliferation response in neonatal rat cardiac fibroblast (FB) induced by angiotensin II (AngII) or epidermal growth factor (EGF), the proximal cytosolic signal transduction pathways which are quite different processes. A phosphorothioate0protected 17-mer directed against the initiation of translation sites P~(42) MAPK mRNA was introduced into FB by liposomal transfection.

Key words: Cardiac fibroblast;Proliferation angiotensin Ⅱ;Epidermal growth factor;Mitogen activated protein kinase;Antisense oligonucleotide;

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Citing This Article：

Ding Bo, Huang Shaoling, Li Yunxia. Inhibitory effects of antisense oligonucleotides targeting mitogen-activated protein kinase (MAPK) mRNA on neonatal rat cardiac fibroblast proliferation induced by AngII and EGF. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).