ISSN 0371-0874, CN 31-1352/Q

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DNA binding activity of the transcription factor HAP is regulated by cellular redox

Yao Jinghong, Xing Yanyan, Wang Zongyang, Zhang Jingliu, Hong Mengmin

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

By using a yeast one-hybrid system, the authors tried to isolate the counterpart of the yeast HAP5 gene from rice cDNA-Gal4 fusion library constructed in a E. coli-yeast shuttle vector pPC86. However, the authors identified a cDNA encoding glutaredoxin. The results indicate that the cellular redox environment of yeast cell may be a factor regulating the CCAAT-box-binding activity of the HAP3 subunit. The results of the HAP3 mutants in which the highly conserved cysteine residues at positions 68 andn 72 had been converted to serine support the above suggestion.

Key words: CCAAT;HAP complex;Transcription factor;Redox system

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Citing This Article:

Yao Jinghong, Xing Yanyan, Wang Zongyang, Zhang Jingliu, Hong Mengmin. DNA binding activity of the transcription factor HAP is regulated by cellular redox. Acta Physiol Sin 1999; 51 (3): (in Chinese with English abstract).