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Modulation of glycine-activated chloride currents by substance P in rat sacral dorsal commissural neurons

Wang Dianshi, Xu Tianle, Li Jishuo

Department of Anatomy and K. K. Leung Brain Research Center,Fourth Military Medical University.Xi^an 710032,Shaanxi；China

Abstract

The modulatory effect of substance P (SP) on strychnine-sensitive glycine (Gly) response was examined in neurons acutely dissociated from the rat sacral dorsal commissural nucleus (SDCN) using nystatin perforated patch recording configuration under voltage-clamp conditions. The facilitatory action of SP on I_(Gly) could also be abolished by pretreatment with chelerythrine or KN-62 in different neurons, a finding suggesting that protein kinase C (PKC) or Ca~(2+)/calmodulin-dependent protein kinase II (CaMKII) possibly contributes to an intracellular pathway of SP in the augmentation of I_(Gly). The results imply that SP may suppress nociception in the spinal cord by potentiating Gly response.

Key words: Substance P;Glycine;Sacral dorsal commissural nucleus;Protein kinase C;Ca~(2+)/calmodulin dependent protein kinase Ⅱ

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Citing This Article：

Wang Dianshi, Xu Tianle, Li Jishuo. Modulation of glycine-activated chloride currents by substance P in rat sacral dorsal commissural neurons. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).