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Cloning and sequencing of cDNA fragment of early responsive gene of rice induced by blast fungus Magnaporthe grisea

Zhou Jianming, Zhu Qun, Bai Yongyan

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032；USA

Abstract

cDNA fragment of early responsive gene (ER1) of rice (Oryza sativa L. subsp. japonica No.4) induced by blast fungus Magnaporthe grisea was isolated using mRNA differential display technique. Northern blot analysis showed that the expression of ER1 gene was induced at an early stage of blast infection. It started to express at 6h, strongly expressed at 8h, weakly expressed at 10h and 12h, and stopped expressed at 16h after blast infection. Southern blot shows that ER1 is one of genes of rice. ER1 gene has been cloned, DNA sequence analysis (219bp) showed no homologue in GenBank of ER1 gene.

Key words: Rice;Magnaporthe grisea;Early responsive gene;mRNA differential display

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Citing This Article：

Zhou Jianming, Zhu Qun, Bai Yongyan. Cloning and sequencing of cDNA fragment of early responsive gene of rice induced by blast fungus Magnaporthe grisea. Acta Physiol Sin 1999; 51 (2): (in Chinese with English abstract).