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Specific cleavages of two different RNAs from TMV and CMV by a di-component ribozyme in vitro

Song Rentao, Zhang Qingqi, LI Jie, Xu Zhengkai

National Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

A hammerhead-based ribozyme RZC was synthesized to cleave specifically a RNA fragment located in the coat protein (CP) gene region of cucumber mosaic virus (CMV) genome. After its cleavage activity being confirmed (lane 5), RZC was further utilized to construct a dicomponent ribozyme, RZ1C with a h ammerhead ribozyme RZ1 specific to the movement protein (MP) gene region of tobacco mosaic virus (TMV) RNA genome. In vitro results indicated that RZ1C was able to cleave both CMV and TMV RNA sequences (lanes 3,6) as efficiently and specifically as its mono-ribozyme counterparts RZ1 and RZ1C.

Key words: Hammerhead ribozyme;Di-component ribozyme;CMV;TMV

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Citing This Article：

Song Rentao, Zhang Qingqi, LI Jie, Xu Zhengkai. Specific cleavages of two different RNAs from TMV and CMV by a di-component ribozyme in vitro. Acta Physiol Sin 1999; 51 (2): (in Chinese with English abstract).