Issue Archive

Heavy metal-responsive genes in kidney bean: Cloning of cDNAs and gene expression analysis

Chai Tuanyao, Zhang Yuxiu, Burkard G

Department of Biology,Graduate School at Beijing,University of Science and Technology of China,Chinese Academy of Sciences.Beijing 100039；France

Abstract

The sequences of the cDNA inserts and homological analysis showed that they encode proline-rich protein (PvSR1), dehydrin (PvSR3), pathogenesis-related protein (PvSR4), polyubiquitin (PvSR5), DnaJ-like protein (PvSR6) and two new HgCl_(2)-stress-related proteins (PvSR2 and PvSR7). Northern blotting analysis revealed that the gene expression of PvSR2 and pathogenesis-related protein were strongly induced in leaf tissue by HgCl_(2) stress, the transcript levels of proline-rich protein, dehydrin, polyubiquitin, DnaJ-like protein and PvSR7 were greatly enhanced too. The various gene products might function to protect cell from heavy-metal stress.

Key words: Kidney bean (Phaseolus vulgaris L.;Heavy metal;DNA sequence and enalysis; Stress-related protein

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Chai Tuanyao, Zhang Yuxiu, Burkard G. Heavy metal-responsive genes in kidney bean: Cloning of cDNAs and gene expression analysis. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).