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Inhibition of GABA-activated currents in isolated rat DRG neurons by bradykinin

Abstract

In the present paper, the modulation of GABA-activated currents by bradykinin (BK) was studied in DRG neurons freshly isolated from rat using whole-cell patch-clamp techniques. Among the neurons (n=34) responding to GABA, 27 were sensitive to BK. Pre-application of BK, eliciting an inward current alone, inhibited GABA-activated current markedly. For example, 10~(-6)mol/L BK suppressed GABA-activated current by about 30%. BK shifted the GABA dose-response curve downward obviously and depressed the maximal response to GABA by 1/3 or more while the K_(d) value was unchanged. The results suggest that BK inhibits GABA-activated current noncompetitively in rat DRG neurons.

Key words: Bradykinin;GABA_(A) receptor;Dorsal root ganglion;Whole-cell patch-clamp techniques

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Citing This Article：

. Inhibition of GABA-activated currents in isolated rat DRG neurons by bradykinin. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).