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Alteration of inositol phosphate metabolism in aortic smooth muscle cells from spontaneously hypertensive rat

Hu Shenjiang, Huang Yuanwei

Cardiovascular Department, the First Affiliated Hospital, Zhejiang Medical University. Hangzhou 310003；China

Abstract

The changes of inositol phosphates in quiescent and GTR_(#gamma#)S activated vascular smooth muscle cells (VSMC) derived from aorta of spontaneously hypertensive rat (SHR) were examined. The inositol phosphate assay revealed that there was mo difference in the inositol-1,4,5-triphosphate (InsP_(3)) level between the quiescent VSMC_(s) of SHR and that of normotensive Wistar-Kyoto rats (WKY). G-protein activator GTR_(#gamma#)S caused a dose-and time-dependent increase in the InsP_(3) level in VSMC_(s). Activated G proteins increased significantly the InsP_(3) level in VSMC_(s) of SHR. In conclusion, the highly sensitive reactivity to GTR_(#gamma#)S in the generationof InsP_(3) in VSMC_(s) from SHR may be related to the possible abnomality of the number, substructure or function of some components in G proteins, phosphoinositide turnover.

Key words: Spontaneously hypertensive rat;Vascular smooth muscle cells;G protein;Inositol phosphates

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Citing This Article：

Hu Shenjiang, Huang Yuanwei. Alteration of inositol phosphate metabolism in aortic smooth muscle cells from spontaneously hypertensive rat. Acta Physiol Sin 1997; 49 (1): (in Chinese with English abstract).