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Endotoxin causes release of calcitonin gene-related peptide(CGRP) from the isolated mesenteric arterial bed in rat

Wang Xian, Wu Zhongxin, Tang Yueming, Han Qide

Institute of Vascular Medicine, The Third Hospital, Beijing Medical University. Beijing 100083

Abstract

In the present study we determined whether ETX could directly trigger release of CGRP from CGRP-containing perivascular nerves in isolated mesenteric arterial bed (MAB) of rat. The results showed that ETX caused time- and concentration (10-100#mu#g/ml) -dependent release of CGRP. ETX(50#mu#g/ml)increased by 20 fold CGRP in perfusate 10-15 min after ETX. Characterization of CGRP in perfusates by reverse-phrase HPLC showed one predominant peak which coeluted with synthetic rat CGRP. Pretreated MAB with capsaicin or ruthenium red inhibited ETX-induced release of CGRP by 90% and 65% respectively. ETX-induced CGRP release was decreased under Ca~(2+)-free perfusion by 80%.

Key words: Release of calcitonin gene-related peptide;Endotoxin;Mesenteric arterial bed

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Citing This Article：

Wang Xian, Wu Zhongxin, Tang Yueming, Han Qide. Endotoxin causes release of calcitonin gene-related peptide(CGRP) from the isolated mesenteric arterial bed in rat. Acta Physiol Sin 1996; 48 (1): (in Chinese with English abstract).