Issue Archive

Effect of U-50, 488H administered centrally in rats on urination and activity of TH-IR neurons in PVH

Di Anke, Huang Long

Department of Physiology, Dalian Medical University. Dalian 116023, Liaoning

Abstract

The effect of a kappa--receptor agonist U--50, 488H on urine flow and the activity of TH--R (tyrosine hydroxylase--immunoreactivity) neurons in PVH (Paraventricular nucleus of the hypothalamus) was studied in ethanol--anesthetized rats. U--50, 488H (4#mu#g/1) #mu#l) injected into PVH induced a potent diuretic response with no significant aetion on mean arterial pressure and heart rate. The diuretic response began within 20 min after injection, reached a peak value 41--60 min later and subsided at about 100 min. this diuretic response could be blocked by pretreatment with kappa--receptor antagonist NBT (Nor--Binaltorphimine Tetrapydrate). Both the number of neurons and staining density of TH--IR in the PVH tended to decrease at 20 min after the injection of U--50, 488H (10#mu#g/10#mul) into the third cerebroventricle. The effect was most evident at 50 min and recovered at about 100 min after injection.

Key words: U-50,488H;NBT;PVH;TH-IR neurons;Urine flow;urinary Na~(+);Urinary K~(+)

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Di Anke, Huang Long. Effect of U-50, 488H administered centrally in rats on urination and activity of TH-IR neurons in PVH. Acta Physiol Sin 1995; 47 (2): (in Chinese with English abstract).