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The catalytic features of peroxidase during tracheary element differentiation of isolated Zinnia mesophyll cells

Huang Xianghui, Liu Shuming, Wang Longhua, Li Rengui, Yan Jiqiong

Department of Biology, East China Normal University. Shanghai

Abstract

Based on an experimental suystem of isolated Zinnia mesophyll cells, this paper describes the features of peroxidase (PO) acting in tracheary elements (TE)differentiation. During the differentiation of TE, lignin content gradually increased (Fig. 1); activities of PO Ⅰ(soluble PO), POⅡ(PO ionically bound with cell wall), POⅢ(PO covalently bound with cell wall)in creased, and the affinity of them to the substrates guaiacol, caryophlfene, caffeic acid and ferulic acid became higher (Fig. 2 and Table 2). The kinetic characteristric of PO inhibition indica that phloroglucinol is a competitive in hibitor, and ammonium ferrous sulfate and dithiothreitol are nonocompetitive ones (Fig. 3). The relationship between PO activity, lignin synthesis and TE fornamtion in TE-inducing culture and TE-inhibiting culture has been dis sed.

Key words: Lignin peroxidase;Inhibitor;Tracheary element;Differentiation;Mesophyll cells;Zinnia elegans

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Citing This Article：

Huang Xianghui, Liu Shuming, Wang Longhua, Li Rengui, Yan Jiqiong. The catalytic features of peroxidase during tracheary element differentiation of isolated Zinnia mesophyll cells. Acta Physiol Sin 1994; 46 (1): (in Chinese with English abstract).