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Modulation of expression of human GM-CSF and GM-CSFR#alpha# by total saponins of Panax ginseng

Wang Shali, Chen Di, Wang Yaping, Liu Yonggang, Jiang Rong

Department of Physiology,College of Basic Medical Sciences,Chongqing Medical University.Chongqing 400016；China

Abstract

Bone marrow stomal cell (BMSC), thymocyte (TC), splenocyte (SC), endothelial cells (EC), and monocyte (MO) conditioned media prepared with TSPG (50 #mu#g/ml) could significantly enhance the proliferation ofo CFU-GM; the expressions of GM-CSF in protein and mRNA level in BMSC, TC, SC, EC and MO induced by TSPG (50 #mu#g/ml) were much higher than that of the control; the expression of GM-CSFR#alpha# protein in hematopoietic cells induced by TSPG (50 #mu#g/ml) was stronger than that of the control; TSPG (50 #mu#g/ml) could stimulate the transient tyrosine phosphorylation of GM-CSFR and Shc protein. We speculate that TSPG may directly and/or indirectly promote the stromal cells and lymphocytes to produce GM-CSF and other cytokine and induce bone marrow hematopoietic cells to express GM-CSF receptors (GM-CSFR#alpha#), leading to the regulation of the GM-CSFR-mediated signals transduction pathway and the proliferation of human CFO-GM.

Key words: Total saponins of Panax;Ginseng;CFU-GM;GM-CSF GM-CSFRα;Signal transduction

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Citing This Article：

Wang Shali, Chen Di, Wang Yaping, Liu Yonggang, Jiang Rong. Modulation of expression of human GM-CSF and GM-CSFR#alpha# by total saponins of Panax ginseng. Acta Physiol Sin 2003; 55 (4): (in Chinese with English abstract).