过刊浏览

中缝背核微量注射L-N-硝基精氨酸甲酯抑制大鼠乙状结肠痛

杨斌, 张励才, 曾因明

徐州医学院江苏省麻醉学重点实验室. 徐州 221002

摘要

用Fos免疫组织化学、烟酰胺腺嘌呤二核苷酸磷酸黄递酶(nicotinamide adenine dinucleotide phosphate-di-aphorase,NADPH-d)组织化学及微量注射技术,观察大鼠乙状结肠注射甲醛(5%)诱发的大鼠乙状结肠炎性痛过程中中缝背核一氧化氮合酶(nitric oxide synthase,NOS)神经元的变化,同时观察中缝背核微量注射L-N-硝基精氨酸甲酯(L-NAME)对乙状结肠痛的调控作用.结果表明,(1)乙状结肠注射甲醛后,大鼠出现明显的内脏痛反应,中缝背核NOS神经元表达明显增多,中缝背核内出现大量Fos蛋白,在整个中缝背核内均有分布,并且出现Fos/NOS双标神经元,约占中缝背核NOS神经元总数的8%,与生理盐水对照组相比差异有显著性;(2)中缝背核注射L-NAME后,可以明显减少乙状结肠炎性痛大鼠的疼痛学评分及脊髓相应节段Fos蛋白.上述结果提示,中缝背核NOS神经元参与调控大鼠乙状结肠痛,NO在中缝背核促进内脏伤害性信息的传递。

关键词： 中缝背核; 乙状结肠; Fos; 一氧化氮合酶

Microinjection of L-NAME into dorsal raphe nucleus inhibits nociceptive response in sigmoid pain model of rats

Yang Bin, Zhang Licai, Zeng Yinming

Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College. Xuzhou 221002,China

Abstract

By means of Fos immunocytochemistry, nicotinamide adenine dinucleotide phosphate - dia-phorase (NADPH-d) histochemistry and microinjection methods, the role of nitric oxide synthase (NOS) ofdorsal raphe (DR) neurons in the modulation of rats sigmoid pain was studied. The results showed: (1 )Rats exhibited aversive behavioral responses related to visceral pain after injecting formalin into the sigmoidwall. NOS neurons in DR were up-regulated, in addition, about 8% of NOS-labeled neurons were Fos posi-tive. By contrast, there was no Fos/NOS double-labeled neurons in the control group. (2) Formalin-in-duced sigmoid pain scores and the expression of Fos in the spinal cord at S1 segment were decreased aftermicroinjecting L-NAME into the DR. These findings suggest that NOS neurons are involved in the modula-tion of formalin-induced sigmoid pain and that NO may play an important role in the transmission of visceralnociceptive message in the midbrain.

Key words: Dorsal raphe;Sigmoid wall;Fos;Nitric oxide synthase

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

杨斌, 张励才, 曾因明. 中缝背核微量注射L-N-硝基精氨酸甲酯抑制大鼠乙状结肠痛[J]. 生理学报 2003; 55 (5): .

Yang Bin, Zhang Licai, Zeng Yinming. Microinjection of L-NAME into dorsal raphe nucleus inhibits nociceptive response in sigmoid pain model of rats. Acta Physiol Sin 2003; 55 (5): (in Chinese with English abstract).