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大鼠海马癫痫电网络重建中爆发式放电神经元的活动

王文挺, 覃星奎, 尹世金, 韩丹

武汉大学医学院生理学系.湖北,武汉 430071；中南民族大学生物医学工程研究所.湖北,武汉 430074

摘要

探讨双侧海马(HPC)神经网络中爆发式放电神经元(BFN)的活动规律及其与海马癫痫网络重建的关系。实验用雄性SD大鼠140只(150--250g),急性强直电刺激(60Hz,2s,0.4--0.6mA)右后背HPC CA1区(ATPDH),同步记录同侧或对侧前背HPC单位放电和深部电图;强直电刺激右前背HPLC(ATADH),同步记录双侧前背HPC单位放电。实验共记录了13.8%(19/138)双侧前背HPC的BFN,其中13个为刺激诱发性BFN,6个为自发性BFN。强直电刺激引起的诱发反应包括:(1)ATPDH明显调制同侧前背HHPC的BFN,产生规则的节律性爆发式放电,刺激后串内动作电位间期(BISI)减小(P<0.001);(2)ATPDH引起对侧前背HPC的BFN出现抑制后轻度调制效应,刺激后动作电位间期(ISI)增大(P<0.001);(3)ATADH后易化对侧前背HPC的自发性BFN节律,增加ISI(P<0.001)和IBI(P=0.01);(4)ATPDH诱导双侧前背HPC的BFN产生规则的节律性爆发式放电,伴有同步或非同步性网络癫痫的形成。

关键词： 癫痫网络; 爆发式放电神经元; 动作电位间期; 强直电刺激; 海马

Electrical activities of bursting-firing neurons in epileptic network reestablishment of rat hippocampus

Wang Wenting, Qin Xingkui, Yin Shijin, Han Dan

Department of Physiology Medical College,Wuhan University.Wuhan 430071,Hubei；China

Abstract

The evoked reactions of the single hippocampal neuron induced by the tetanization mainly included: (1) the firing patterns of the BFNs in ipsilateral anterior dorsal HPC were obviously modulated by the ATPDH from tonic firing into rhythmic bursting. The bursting interspike intervals (BISI) decreased. (2)There were mild modulations of the firing patterns of the BFNs in contralateral anterior dorsal HPC following post-inhibition of the firing rate of single neuron induced by the ATPDH. The interspike intervals (ISI) increased obviously. (3)Post-facilitation of rhythmic bursting-firing of the BFNs in contralateral anterior dorsal HPC was induced by ATADH; both the ISI and the IBI increased. (4) Synchronous or asynchronous rhythmic bursting-firing of the BFNs and the network epileptiform events ipsilateral or contralateral anterion dorsal HPC were elicited by the ATPDH.

Key words: Epileptic network;Bursting-firing neuron;Intersipke interval;Tetanization;Hippocampus

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引用本文：

王文挺, 覃星奎, 尹世金, 韩丹. 大鼠海马癫痫电网络重建中爆发式放电神经元的活动[J]. 生理学报 2003; 55 (6): .

Wang Wenting, Qin Xingkui, Yin Shijin, Han Dan. Electrical activities of bursting-firing neurons in epileptic network reestablishment of rat hippocampus. Acta Physiol Sin 2003; 55 (6): (in Chinese with English abstract).