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Janus kinase 3 facilitates the migration of breast cancer cells by store-operated calcium channel

LIU Xia^1,2, WEI Ting^1,2, GAO Zhao-Di^1,2, ZHAO Xiu-Liang^1,2, WU Hui-Qing^1,2, YAN Jing^1,2,*

¹Department of Physiology, Institute of Basic Medicine, Jining Medical University, Jining 272067, China；²Department of Physiology, Institute of Basic Medicine, Xinxiang Medical University, Xinxiang 453003, China

Abstract

The present study was aimed to investigate the effect of Janus kinase 3 (JAK3) on the migration of breast cancer cells and the underlying mechanism. The expression of JAK3 in breast cancer MCF-7 cells was silenced by siRNA (siJAK3). The migration ability of MCF-7 cells was detected by scratch test. The activity of store-operated calcium channel (SOCC) was detected by fluorescence calcium imaging. The expression levels of Orai1 and STIM1, key molecules in the process of store-operated calcium entry (SOCE) were detected by Western blot and RT-PCR. The results showed that 2-APB, an inhibitor of SOCC, could inhibit the migration ability of MCF-7 cells. siJAK3 transfection significantly inhibited the migration ability of MCF-7 cells, decreased the activity of SOCC, and down-regulated mRNA and protein expression levels of Orai1 and Stim1. Over-expression of Orai1 or STIM1 in JAK3-silenced cells restored their migration ability. These results suggest that JAK3 facilitates the migration of breast cancer cells by SOCC.

Key words: Ca2+; store-operated calcium entry; Orai1; Janus kinase 3

Received: 2019-02-06　　Accepted: 2019-07-01

Corresponding author: 严静　　E-mail: yanjing102@mail.jnmc.edu.cn

DOI: 10.13294/j.aps.2019.0075

Citing This Article：

LIU Xia, WEI Ting, GAO Zhao-Di, ZHAO Xiu-Liang, WU Hui-Qing, YAN Jing. Janus kinase 3 facilitates the migration of breast cancer cells by store-operated calcium channel. Acta Physiol Sin 2019; 71 (6): 874-882 (in Chinese with English abstract).