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Effects of different concentrations of calcitonin gene-related peptide on long-term depression of hippocampus in mice

WU Xin, WANG Dong-Hui, SUN Ning-Ning, SU Shao-Hua, ZHANG Song-Jiang, GAO Jian-Feng^*

Department of Physiology, School of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China

Abstract

The purpose of this study was to explore the effects of calcitonin gene-related peptide (CGRP) on the long-term depression (LTD) of hippocampus in mice. Sixty C57BL/6J mice (30 days old) were randomly divided into control group, three CGRP (50, 100, and 200 nmol/L) groups, CGRP + CGRP8-37 group and CGRP + APV group (10 mice for each group). The effects of exogenous application of different concentrations of CGRP on synaptic plasticity and LTD in hippocampus of mice were detected by in vitro recording of local field potential. The results showed that higher doses (100 and 200 nmol/L) of CGRP significantly enhanced the induction of LTD in the hippocampus. Moreover, CGRP increased the magnitude of N-methyl-D-aspartate (NMDA) receptor-mediated excitatory postsynaptic currents. The above-mentioned effects of CGRP were blocked by either CGRP selective antagonist CGRP8-37 or NMDA receptor antagonist APV. These results suggest that CGRP can dose-dependently enhance the induction of LTD in hippocampus of mice, and the underlying mechanism involves the mediation of NMDA receptor function. 

Key words: Calcitonin gene-related peptide; hippocampus; long-term depression; glutamate receptor

Received: 2019-03-12　　Accepted: 2019-05-28

Corresponding author: 高剑峰　　E-mail: jc.gjfeng@hotmail.com

DOI: 10.13294/j.aps.2019.0076

Citing This Article：

WU Xin, WANG Dong-Hui, SUN Ning-Ning, SU Shao-Hua, ZHANG Song-Jiang, GAO Jian-Feng. Effects of different concentrations of calcitonin gene-related peptide on long-term depression of hippocampus in mice. Acta Physiol Sin 2019; 71 (6): 839-845 (in Chinese with English abstract).