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[Effects of neonatal isolation stress on hyperlocomotion of complexin II knockout mouse.] [Article in Chinese]

YU Huan^*, ZHAO Yong, HE Wei, LI Xing-Nuan, ZHOU Yi-Chun, LIU Li-Ping

Department of Physiology and Pathophysiology, Basic Medical School of Jiujiang University, Jiujiang 332000, China

Abstract

The aim of this study was to investigate the influence of neonatal isolation stress on hyperlocomotion in complexin Ⅱ knockout mouse (Cplx2−/−). The mice were randomly divided into 4 groups: Cplx2−/− with stress, Cplx2+/+ with stress, Cplx2−/− without stress and Cplx2+/+ without stress. Isolation stress was employed on the pups of stress groups from the 2nd day after the postnatal to the 21st day. The PCR was used to determine the gene type and the hyperlocomotion test was employed to detect the change of animal behavior after methamphetamine or saline injection (i.p.). The results showed that the animals of all groups increased their movement after injection of 0.2 mg/kg methamphetamine in different levels (P < 0.01), compared with those injected with saline. The Cplx2−/− mouse with stress revealed a significant increase in the distance of free movement after injection of 0.2 mg/kg methamphetamine compared with the knockout mouse without stress (P < 0.001). When Cplx2−/− mouse with stress was compared with wild type with stress, Cplx2−/− mouse with stress had more movement (P < 0.001), indicating that Cplx2 has effect on the hyperlocomotion as well. These results suggest an involvement of stress and Cplx2 in the movement behavior of mice.

Key words: maternal deprivation stress; Cplx2; methamphetamine; hyperlocomotion

Received: 2011-01-21　　Accepted: 2011-03-18

Corresponding author: 于欢　　E-mail: huanyu@msn.com

Citing This Article：

YU Huan, ZHAO Yong, HE Wei, LI Xing-Nuan, ZHOU Yi-Chun, LIU Li-Ping. [Effects of neonatal isolation stress on hyperlocomotion of complexin II knockout mouse.] [Article in Chinese]. Acta Physiol Sin 2011; 63 (4): 300-304 (in Chinese with English abstract).