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Effect of bilateral injection of calcitonin gene-related peptide into amygdala on learning and memory of mice

WU Xin, ZHENG Wan-Jun, LV Ming-Hui, GAO Jian-Feng^*

Department of Physiology, School of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China

Abstract

The aim of the present study was to explore the effects of different doses of calcitonin gene-related peptide (CGRP) injected into the central nucleus of amygdala on cognitive function, learning and memory of mice. C57BL/6J mice (30 days old) were randomly divided into control, sham, and three CGRP groups (10 mice for each group). Three doses of CGRP (200, 400 and 800 ng) were bilaterally administered into the central nucleus of the amygdala. Open field test was used to assess cognitive function. Novel object recognition and Morris water maze test were used to evaluate learning and memory of the mice. The results of open field test showed that 800 ng CGRP significantly increased the locomotive score. The results of novel objective recognition test showed that 400 ng CGRP significantly increased the recognition index. Compared with control group, 400 and 800 ng CGRP groups showed significantly shortened latency period and increased crossing times. Simultaneously, the latency periods of 400 and 800 ng CGRP groups were shorter than that of 200 ng CGRP group. These results suggest that bilateral injection of CGRP into amygdala dose- dependently enhances the learning and memory function of mice.

Key words: Calcitonin gene-related peptide; central nucleus of amygdala ; learning and memory ; synaptic plasticity

Received: 2016-08-30　　Accepted: 2016-10-17

Corresponding author: 高剑峰　　E-mail: jc.gjfeng@hotmail.com

Citing This Article：

WU Xin, ZHENG Wan-Jun, LV Ming-Hui, GAO Jian-Feng. Effect of bilateral injection of calcitonin gene-related peptide into amygdala on learning and memory of mice. Acta Physiol Sin 2017; 69 (2): 167-171 (in Chinese with English abstract).