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Effects of diazoxide on Fas/FasL protein expressions in rat myocardium suffered from long--term hypothermic preservation

Fan Ying, Zheng Mingzhi, Guo Wei, Jiang Jianping, Zhu Li, Shen Yueliang, Chen Yingying

Department of Physiology, School of Medicine, Zhejiang University.Hangzhou 310058,Zhejiang；China；Department of Pathology, Shanghai University of Traditional Chinese Medicine.Shanghai 201203

Abstract

The purpose of this study was to investigate the effect of a mitochondrial ATP-sensitive potassium channel （mitoK_(ATP)） opener, diazoxide （DE）, on Fas/FasL protein expressions in rat heart suffered from long-term hypothermic preservation. The Langendorff isolated rat heart model was used. The hearts were stored in 4℃ Celsior solution with or without （control） DE for 8 h followed by 60 min of reperfusion. The recovery of rate-pressure product （RPP） was observed. Apoptotic cardiomyocytes were detected by TdTmediated dUTP nick end labeling （TUNEL） technique. The expressions of Fas/FasL proteins were also analyzed by immunohistochemical method. The results showed that compared with the control group, DE （30 mmol/L） increased the recovery of RPP during reperfusion, reduced the percentage of apoptotic cells and the expressions of Fas and FasL proteins in rat hearts suffered from 8 h of hypothermic preservation. The above effects of DE were attenuated by a mitoK_(ATP) channel inhibitor 5-hydroxydecanoate （5-HD）. These results indicate that DE could alleviate rat myocardial injury induced by ischemia-repeffusion through reducing the expressions of Fas and FasL proteins via opening of mitoK_(ATP) channel.

Key words: Diazoxide;Fas/FasL;mitochondrial ATP-sensitive potassium channel;heart preservation;cardioprotection

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Citing This Article：

Fan Ying, Zheng Mingzhi, Guo Wei, Jiang Jianping, Zhu Li, Shen Yueliang, Chen Yingying. Effects of diazoxide on Fas/FasL protein expressions in rat myocardium suffered from long--term hypothermic preservation. Acta Physiol Sin 2008; 60 (1): 11-16 (in Chinese with English abstract).