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Effect of lysophosphatidic acid on differentiation of embryonic neural stem cells into neuroglial cells in rats {sl in vitro}

Cui Huilin, Qiao Jiantian

Department of Neurobiology,Shanxi Medical University.Taiyuan 030001,Shanxi

Abstract

To study the effect of lysophosphatidic acid (LPA) on the differentiation of embryonic neural stem cells (NSCs) into neuroglia cells in rats in vitro, both oligodendrocytes and astrocytes were detected by their marker proteins galactocerebroside (Gal-C) and glia fibrillary acidic protein (GFAP), respectively, using double-labeling immunocytochemistry. RT-PCR assay was also used for analyzing the expression of LPA receptors in NSCs. Our results showed that: (1) Different concentrations of LPA (0.05-3.0 #mu#mol/L) were added to culture media and cell counting was carried out on the 7th day in all groups. Exposure to LPA led to a dose-dependent increase of oligodendrocytes with the response peaked at 1.0 #mu#mol/L, with an increased percentage of 32.6% (P<0.01) of total cells as compared to that of 8.5% in the vehicle group; (2) LPA showed no effect on the differentiation of NSCs into astrocytes; (3) RT-PCR assay showed that LPA_(1) and LPA_(3) receptors were strongly expressed while LPA_(2) receptor expressed weakly in NSCs. These results suggest that LPA at low concentration might act as an extracellular signal through the receptors in NSCs, mainly LPA_(1) and LPA_(3) receptors, to promote the differentiation of NSCs into oligodendrocytes, while it exhibits little, if any, conceivable effect on the differentiation of NSCs into astrocytes.

Key words: Differentiation;neuroglial cells;neural stem cells;Lysophosphatidic acid;lysophosphatidic acid receptors

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Citing This Article：

Cui Huilin, Qiao Jiantian. Effect of lysophosphatidic acid on differentiation of embryonic neural stem cells into neuroglial cells in rats {sl in vitro}. Acta Physiol Sin 2007; 59 (6): 759-764 (in Chinese with English abstract).