Issue Archive

Regulative effects of ovarian steroids on rat gastric motility and sensitivity

Yang Xia, Liu Ran, Dong Yan

Qilu Hospital, Shandong University.Jinan 250012,Shandong

Abstract

Women often complain more gut symptoms during pregnancy and the luteal phase of the menstrual cycle. To investigate the relationship of ovary steroids with the abnormal gut motility and sensitivity, the expression of cholecystokinin (CCK), calcitonin gene-related peptide (CGRP) and their receptors in rat stomach were studied in ovariectomized rats. Blood samples were collected for estradiol (E_(2)), progesterone (P_(4)), CCK and CGRP radioimmunoassay; Expression of CCK_(A) receptor in fundus was assessed by Western blotting and CGRP receptor was determined by ~(125)I-CGRP radioligand binding assay (RBA). The replacement therapy with estradiol benzote (EB) could dose-dependently increase the plasma CCK level and the expression of gastric CCK_(A) receptor (P<0.05 respectively). P_(4) replacement therapy could stimulate the release of CGRP and increase the binding sites of CGRP receptors in stomach (P<0.05 respectively). The combined effect of EB and P_(4) was to stimulate the release of CCK and CGRP, increase the expression of gastric CCK_(A) and CGRP receptors. These results indicated that EB could inhibit gastric emptying by increasing CCK secretion and CCK_(A) receptor expression in ovariectomized rats. P_(4) could increase gut sensitivity by up-regulating the release of CGRP and the activity of CGRP receptor. It could be deduced from these observations that CCK_(A) and CGRP receptor antagonists could be used for female patients who suffer from gastrointestinal dysfunction closely related with the menstrual cycle, such as distension, satiety, bloating and abdominal pain.

Key words: Estradiol;Progesterone;Receptor;Cholecystokinin;calcitonin gene-related peptide

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Yang Xia, Liu Ran, Dong Yan. Regulative effects of ovarian steroids on rat gastric motility and sensitivity. Acta Physiol Sin 2006; 58 (3): (in Chinese with English abstract).