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Neuroprotective effect of lysophosphatidic acid on A#beta#P31-35-induced apoptosis in cultured cortical neurons

Zheng Zhaoqing, Fang Xianjun, Zhang Yu, Qiao Jiantian

Department of Neurobiology, Shanxi Medical University. Taiyuan 030001, Shanxi, China；Virginia Commonwealth University, Department of Biochemistry. Richmond, VA 23298, USA

Abstract

It has been reported that lysophosphatidic acid (LPA) at its lower concentrations prevents apoptosis induced by serumdeprivation in cultured cortical neurons when LPA is added into the cultural medium with serum withdrawal. The present study was designed to investigate whether LPA could also block the apoptosis induced by #beta#-amyloid peptide fragment 31-35 (AβP31-35) in cultured cortical neurons by using techniques of DNA fragmentation electrophoresis, HO33342 staining, and TUNEL examinations. The results showed that pretreatment of LPA suppressed the A#beta#P31-35-induced apoptosis only when LPA was applied to the cultured neurons with lower concentrations (1～10 #mu#mol/L) and especially, with a preceding time of 12～24 h before the A#beta#P31-35 exposure. These facts imply that LPA also acts as a neuroprotective factor against A#beta#P31-35-induced apoptosis, though the mechanism underlying the protective action in this case may be more complex than that involved in the serum deprivation-induced apoptosis.

Key words: Cultured cortical neurons;Apoptosis;#beta#-amyloid peptide fragment 31-35;Lysophosphatidic acid;Neuroprotective effect;Mice

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Citing This Article：

Zheng Zhaoqing, Fang Xianjun, Zhang Yu, Qiao Jiantian. Neuroprotective effect of lysophosphatidic acid on A#beta#P31-35-induced apoptosis in cultured cortical neurons. Acta Physiol Sin 2005; 57 (3): (in Chinese with English abstract).