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Adenosine protects cardio myocytes from hypoxia/ reoxygenation injury

WANG Xinyang , ZHOU LiLong, DING Jia- Wang, FENG Yi-Bai, CHENG Long- Xian

Department of Cardiology, Union Hospital Affiliated to Tongji Medical College , Huazhong Univesity of Science and Technology, Wuhan430022； Yichang People Hospital , Yichang 443000。

Abstract

The aim of this study was to investigate the protective effect of adenosine(ADO) on car diomyocytes following hypoxia/ reoxygenation(H/ R) and its molecular mechanism.Primary cultured cardiomyocytes of neonatal rats were divided into two groups，namely H/ R(control) and ADO(1 .0p mol/ L) groups .The morphologic changes in cardiomyocytes were observed under an inverted phasecontrast microscope .The following parameters of the two groups were determined:lactate dehydrogenase(LDH) activity，intracellular calcium concentration and malondialdehyde(MDA) content .Tumor necrotic factor(TNF-a) assay was performed using an ELISA kit and NF KB in the nucleus was analyzed
by electrophoretic mobility shift assay(E MS A) .The results are as follows:(1)after H/ R injury，cardiomyocytes contracted，tending to get round in shape and its pseudopods decreased，while marked morphological changes were not observed in ADO group;(2) LDH leakage maintained at a lower level in
ADO group than that in the control group during H/ R(both P<0. 01);(3) ADO significantly reduced the concentration of calcium in cells and prevented calcium overload during H/ R(both P<0. 01);(4)ADO markedly reduced the content of MDA during H/ R(both P<0.01);(5) ADO inhibited the production of TNF-a during H/ R(both P<0. 01);and(6) ADO dower regulated NF KB binding activity of
cardiomyocytes during H/ R(both P<0.01)。The results suggest that(1)exogenous ADO attenuates H/ R injury of cultured cardiomyocytes;(2) exogenous ADO inhibits the production of TNF-a after H/ R injury;(3) exogenous ADO prevents the activation of NF-KB，which may be the molecular mechanism of wirregulation of TNF-a expression.

Key words: adenosine;hypoxia/ reoxygenation injury;cardio myocytes

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Citing This Article：

WANG Xinyang , ZHOU LiLong, DING Jia- Wang, FENG Yi-Bai, CHENG Long- Xian. Adenosine protects cardio myocytes from hypoxia/ reoxygenation injury. Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).