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EFFECT OF IGF-II ON PROGESTERONE PRODUCTION BY CULTURED RAT LUTEAL CELLS IN VITRO

Ni JIANG, Zhu hui, Zhang Wei, WANG JIN-HUA, MA QING-NIAN

Department of Physiology, Harbin Medical University ，Harbin 150086)

Abstract

It has been reported recently that IGF- Ⅱ( plays an important role in the regulation of ovarian function. The aim of this study is to investigate whether IGF-Ⅱ( can affect progesterone production by cultured rat luteal cells. The results showed that IGF- Ⅱ(could significantly stimulate basal progesterone production in a dose-dependant manner without altering the cAMP content. IGF- Ⅱ could enhance 3H-leu incorporation into protein and 3H-TdR incorporation into DNA of the luteal cells，but these effects could be
inhibited by CYX and Act D. The above results suggest that stimulating effect of IGFⅡ( on progesterone production by rat luteal cells may be related to enhanced protein and DNA synthesis.

Key words: insulin like growth factor-Ⅱ (IGF-Ⅱ);Progesterone; luteal cells; ~（3）H-leu;

Received: 　　Accepted:

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Citing This Article：

Ni JIANG, Zhu hui, Zhang Wei, WANG JIN-HUA, MA QING-NIAN. EFFECT OF IGF-II ON PROGESTERONE PRODUCTION BY CULTURED RAT LUTEAL CELLS IN VITRO. Acta Physiol Sin 1996; 48 (5): (in Chinese with English abstract).