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Electrophysiological effects of resveratrol on guinea pig papillary muscles

Zhao Juan, Ma Huijie, Dong Jinghui, Zhang Liping, Liu Hualei, Wang Qingshan

Department of Physiology, Institute of Basic Medicine, Hebei Medical University.Shijiazhuang 050017,Hebei

Abstract

The purpose of this study was to investigate the electrophysiological effects of resveratrol on guinea pig papillary muscles and the underlying mechanism. Action potentials were recorded using intracellular microelectrode technique. The results obtained are as follows: (1) In normal papillary muscles, resveratrol (30, 60, and 120 #mu#mol/L) shortened the duration of action potential (APD) in a concentration-dependent manner; (2) In partially depolarized papillary muscles, resveratrol (60 #mu#mol/L) not only shortened APD, but also decreased the amplitude of action potential (APA), overshoot (OS) and maximal rate of depolarization in phase 0 (V_(max)); (3) Perfusion with Ca~(2+)-free K-H solution, completely abolished the effects of resveratrol (60 #mu#mol/L) on papillary muscles; (4) Application of potassium channel blocker tetraethylammonium chloride (TEA, 20mmol/L) did not prevent the effect of resveratrol (60 祄ol/L) on action potential; (5) Pretreatment with N~(G)-nitro-L-arginine methyl ester (L-NAME, 1 mmol/L), a nitric oxide (NO) synthase inhibitor, failed to abolish the effect of resveratrol (60 #mu#mol/L). All these results indicate that the electrophysiological effects of resveratrol on guinea pig papillary muscles are likely due to the reduction in calcium influx, which might not be mediated by NO.

Key words: electrophysiology;resveratrol;Action potential;Papillary muscle;calcium current

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Citing This Article：

Zhao Juan, Ma Huijie, Dong Jinghui, Zhang Liping, Liu Hualei, Wang Qingshan. Electrophysiological effects of resveratrol on guinea pig papillary muscles. Acta Physiol Sin 2004; 56 (6): (in Chinese with English abstract).