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EFFECT OF MOTILIN ON CONTRACTILE ACTIVITY OF ISOLATED SMOOTH MUSCLE OF STOMACH

ZHOU LU , WANG XIN

Department of Physiology, Institute of Basic Medical Sciences CAMS and School of Basic Medicine PUMC, Beijing 100005)

Abstract

The present study was conducted to investigate the contractile effect of motilin on isolated smooth muscle cells of rat stomach. The results were as follows:(1)Motilin elicited contraction of gastric smooth muscle cells in a dose-dependent manner from 10一“to 10一’“mol.(2)At concentration of 10一‘“mol，the contractile response of antral smooth muscle cells exceeded that of the body and pyrous muscle cells. (3) Arlti-motilin serum could completely eliminate the stimulatory effect of motilin，which was not affected by atropine，TTX，cimetidine or loxiglumide.(4)TMB- 8，an inhibitor of intracellular Ca2+ release，could also suppress completely the motilin effect.
The above results suggested that the motilin effect on isolated gastric smooth muscle cells is a direct one，being mediated by highly specific motilin receptors in connection with the event of intracellular release of Ca2+.

Key words: motilin;Contraction;isolated gastric smooth muscle cells; TMB-8

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Citing This Article：

ZHOU LU , WANG XIN. EFFECT OF MOTILIN ON CONTRACTILE ACTIVITY OF ISOLATED SMOOTH MUSCLE OF STOMACH. Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).