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BOVINE SERUM ALBUMIN-CONJUGATED CORTICOSTERONE INHIBITS THE RELEASE OF ARGININE VASOPRESSIN

Liu Xiu, Wang Chunan, Chen Yizhang

Department of Physiology, Second Military Medical University, Shanghai 200433

Abstract

The arginine vasopressin (AVP) released from rat hypothalamic slices containing paraventricular and supraoptic nuclei sectioned with vibratome and incubated in mrcrochambers was measured by radioimmunoassay. The effect of bovine serum albuminconjugated corticosterone (B-BSA) on the AVP release was investigated. The results were as follows:(1)B-BSA，within 20 min，inhibited the AVP release in a dose-dependent manner from 10一7to 10一4 mol/L. (2) The inhibitory effect of B-BSA was partially blocked by RU486(10一4一10一“mol/L).(3) With the elevation of Call in the incubation medium the inhibitory effect of B-BSA was enhanced. (4)The inhibr troy effect of B-BSA was also enhanced in the presence of neomycin(10~(4)一10~(3)mol/L).These results suggested that glucocorticoid，without entering into cells，could inhibit AVP release from rat hypothalamic slices. This effect might be mediated via a
non-genomic mechanism involving a change of CaZ+ influx across the cell membrane.

Key words: hypothalamic slice;Arginine vasopressin;bovine serum albumin-conjugated corticosterone;RU486;calcium

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Citing This Article：

Liu Xiu, Wang Chunan, Chen Yizhang. BOVINE SERUM ALBUMIN-CONJUGATED CORTICOSTERONE INHIBITS THE RELEASE OF ARGININE VASOPRESSIN. Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).