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CYTOPROTECTIVE EFFECT OF EPIDERMAL GROWTH FACTOR ON CULTURED RABBIT AIRWAY EPITHELIAL CELLS EXPOSED TO OZONE

QIN XIAO-QUN, SUN XIU-HONG, LOU ZI-QIANG , GUAN CHA-XIANG , ZHANG CHANG-QING

(Department of Physiology,Human Medical University, Changsha 410078)

Abstract

In this study，it was observed that ozone (03) exposure has cytotoxic effects on cultured airway epithelial cells，which was positively related with exposure duration. Both the production of malondialdehyde (MDA) and the 3H release in the exposure group were much higher than the control (P<0. 01)，suggesting that lipoperoxidation occurring in the cell membrane was reponsible for the cellular injury observed under 03 attack. EGF at low concentration (5 ng/ml) showed cytoprotective effect on airway epithelial cells exposed to 03 as shown' by attenuated MDA production and decrease of 3H release and cytotoxic index (P<0. 0l).Pretreatment with EGF could reverse the depletion of intracellular GSH content as a result of 03 exposure and increase the total glu
tathionein the cells. The above results suggest that the cytoprotection of EGF on airway epithelial cells may be related to its promotive effect on GSH synthesis.

Key words: airway epithelial cells;ozone;;glutathione; malondialdehyde ;

Received: 　　Accepted:

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Citing This Article：

QIN XIAO-QUN, SUN XIU-HONG, LOU ZI-QIANG , GUAN CHA-XIANG , ZHANG CHANG-QING. CYTOPROTECTIVE EFFECT OF EPIDERMAL GROWTH FACTOR ON CULTURED RABBIT AIRWAY EPITHELIAL CELLS EXPOSED TO OZONE . Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).