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The effect of acetylcholine on the proliferation and apoptosis of three kinds of cultured human pituitary adenoma cells

Chi Sumin, Li Chengxin, Liu Yali, Zhu Yunlong, Gu Jianwen, u Liang, Wang Fuzhou

Department of Physiology,the Fourth Military Medical University.Xi^an 710032,Shaanxi；China；Department of Neurosurgery,Xijing Hospital,the Fourth Military Medical University.Xi^an 710032,Shaanxi

Abstract

The results showed that (1) the positive staining of ChAT was obviously observed in the cells of the three kinds of human pituitary adenoma, however, it was lower than that in normal human pituitary gland; (2) ACh had a similar effect on the proliferation of the three kinds of human pituitary adenoma cells. ACh at 0.1-10 #mu#mol/L decreased the [~(3)H] TdR incorporation and the MTT A value in a dose-dependent manner. At the same time, ACh decreased the ratio of S or G_(2) phase pituitary adenoma cells significantly, but increased the ratio of G_(1) phase pituitary tumour cells markedly; (3) the effect of acetylcholine on the proliferation of human pituitary adenoma cells was inhibited by atropine, but not by tubocurarine; (4) ACh had no effect on the apoptosis of human pituitary adenoma cells cultured in vitro. These data suggest that ACh may have a significant modulating effect on the proliferation of pituitary adenoma cells by means of paracrine or autocrine, and the effect is mediated by muscarinic receptor.

Key words: Acetylcholine;Pituitary gland;Adenoma;Proliferation;Apoptosis

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Citing This Article：

Chi Sumin, Li Chengxin, Liu Yali, Zhu Yunlong, Gu Jianwen, u Liang, Wang Fuzhou. The effect of acetylcholine on the proliferation and apoptosis of three kinds of cultured human pituitary adenoma cells. Acta Physiol Sin 2002; 54 (3): (in Chinese with English abstract).