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Transformation of choline dehydrogenase gene and identification of salt-tolerance in transgenic tomato

Wang Shufang, Wang Junling, Zhao Yanxiu, Zhang Hui

Key Laboratory of Plant Stress,Shandong Normal University.Jinan 250014,Shandong

Abstract

The betA gene which encodes choline dehydrogenase that converts choline into glycinebetaine has been cloned from Escherichia coli TG1. Agrobacterium-mediated transformation has been used to introduce the betA gene under the control of the 35S promoter of cauliflower mosaic virus as well as the npt II marker gene into tomato (Lycopersicon esculentum). Salt tolerance assays were performed on two independent transgenic plants. Under salt stress, the osmotic adjustment ability of the transgenic tomato was higher than that of the control plant. At 200 mmol/L NaCl, there is no visible symptoms of salt damage in transgenic tomato whereas the lower leaves of the control plant turned yellow and exhibited necrosis. The results suggest a positive effect of the betA gene on the level of salt tolerance in transgenic plants.

Key words: Choline dehydrogenase gene( betA);Transgenic tomato;Salt tolerance

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Citing This Article：

Wang Shufang, Wang Junling, Zhao Yanxiu, Zhang Hui. Transformation of choline dehydrogenase gene and identification of salt-tolerance in transgenic tomato. Acta Physiol Sin 2001; 53 (3): (in Chinese with English abstract).