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Analysis of two antisense transgenes inhibiting expression of their endogenous genes in transgenic tomatoes

Ye Zhibiao, Li Hanxia

Department of Huazhong Agricultural University, Wuhan 430070,Hubei

Abstract

The activities of ethylene forming enzyme(EFE), polygalacturonase(PG) and pectinesterase(PE),and ethylene evolution were measured in the fruits and leaves of two antisense transgenic lines of tomato.The results show that in comparison with the wild type,ethylene evolution was specifically inhibited in the leaves(5 times lower)and fruits(12 time lower) of lines carrying the antisense EFE transgene, and only PG activity was reduced to 32% or 52% in fruits of the lines with antisense PG transgene. The EFE activities in fresh leaves and fruits were grealy inhibited and remained lower during fruit storage.The expression of endogenous genes in transgenic tomato plants was significantly down-regulated.

Key words: Tomato;Antisense gene;Ethylene forming enzyme;Polygalacturonase

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Citing This Article：

Ye Zhibiao, Li Hanxia. Analysis of two antisense transgenes inhibiting expression of their endogenous genes in transgenic tomatoes. Acta Physiol Sin 1996; 48 (2): (in Chinese with English abstract).