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Protective effect of endothelium-derived relaxing factor on ischemic (hypoxic) and reperfused (reoxygenated) myocardium

Wan Mei, Yu Zhanjiu

Pathophysiology Laborotory, Hebei Academy of Medical Sciences. Shijiazhuang 050021, Hebei

Abstract

When heart perfusion was stopped for 30 min and reinstitued for 20 min with K-H buffer containing NO, the coronary flow rate(CFR), left ventricular pressure(LVP) and ±dp/dt_(max) increased significantly. When NO was replaced by L-NNA opposite effects were observed. L-Arg alone was without effect on CFR, LVP and ±dp/dt_(max), but attenuated the decreasing effect of L-NNA on CFR. NO decreased MDA and NAGase content of myocardium while L-NNA increased them. When cultured ventricular myocytes were subjected to hypoxia for 30 min and reozygenated for 20 min, none of the substances under investigation showed any effects on Ca~(2+)content of heart cells, but all of them decreased MDA, NAGase content of the culture tissue after reoxygenation. NO plays an important role in protecting myocardium from is chemic and reperfused injury by improving blood supply of reperfused myocardium and attenuation of oxygen free radical injury.

Key words: Nitric oxide;Myocardial ischemia and reperfusion injury;Myocardial hypoxia and re oxygenation injury;Langendorff heart;Myocyte;Oxygen free radical

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Citing This Article：

Wan Mei, Yu Zhanjiu. Protective effect of endothelium-derived relaxing factor on ischemic (hypoxic) and reperfused (reoxygenated) myocardium. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).