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Effect of acetylcholine on the proliferation of T lymphocyte of rat spleen

Qiu Yihua, Peng Yuping, Zhang Qingquan, Wang Jianhe

Department of Physiology, Nantong Medical College. Nantong 226001, Jiangsu

Abstract

ACh at 10~(-9)mol/L to 10~(-4)mol/L range significantly enhanced the proliferation of T cells, reaching a maximum at 10~(-7)and 10~(-6)mol/L. The proliferation of lymphocytes first stimulated by various concentrations of ACh (10~(-9), 10~(-7) or 10~(-5)mol/L) for one hour and incubated with Con A for 72h still showed increase. When ACh was washed off for one hour, the proliferative enhancement of lymphocytes to Con A remained to occur. However, ACh did not enhance the proliferation of T cells which were once stimulated with Con A for 6h. Atropine at 10~(-7) or 10~(-6)mol/L abolished completely the effect of ACh at 10~(-9)or 10~(-5)mol/L. The action of ACh at 10~(-7)mol/L could be blocked by 10~(-6)mol/L atropine. These data suggest that ACh at a wide range of concentrations can enhance cellular immunity, an effect occurring either before or after T cells are activated through muscarinic cholinergic receptors.

Key words: Acetylcholine;T-lymphocyte;Neurotransmitter;Cellular immunity;Neuroimmunomod-ulation

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Citing This Article：

Qiu Yihua, Peng Yuping, Zhang Qingquan, Wang Jianhe. Effect of acetylcholine on the proliferation of T lymphocyte of rat spleen. Acta Physiol Sin 1995; 47 (3): (in Chinese with English abstract).