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[Palmitate induces apoptosis and endoplasmic reticulum stress in human umbilical cord-derived mesenchymal stem cells.] [Article in Chinese]

LU Jun, DONG Hui-Yue, LIN Ling-Jing, WANG Qing-Hua, HUANG Liang-Hu^*, TAN Jian-Ming^*

Fujian Provincial Key Laboratory of Transplant Biology, Fuzhou General Hospital, Fuzhou 350025, China

Abstract

The saturated free fatty acid (FFA), palmitate, could induce apoptosis in various cell types, but little is known about its effects on human umbilical cord-derived mesenchymal stem cells (hUC-MSCs). Here, we investigated whether palmitate induced apoptosis and endoplasmic reticulum (ER) stress in hUC-MSCs. hUC-MSCs were stained by labeled antibodies and identified by flow cytometry. After administration with palmitate, apoptotic cell was assessed by flow cytometry using the Annexin V-FITC/7-AAD apoptosis detection kit. Relative spliced XBP1 levels were analyzed using semi-quantitative RT-PCR. The mRNA of BiP, GRP94, ATF4 and CHOP were analyzed by real-time PCR. Relative BiP and CHOP protein were analyzed using Western blot analysis. The results showed that hUC-MSCs were homogeneously positive for MSC markers; palmitate increased apoptosis of hUC-MSCs and activated XBP1 splicing, BiP, GRP94, ATF4 and CHOP transcription. These findings suggest that palmitate induces apoptosis and ER stress in hUC-MSCs.

Key words: palmitate; mesenchymal stem cells; apoptosis; ER stres

Received: 2013-04-27　　Accepted: 2013-08-05

Corresponding author: 黄梁浒,谭建明　　E-mail: hlhtiger@yahoo.com.cn,tanjm156@yahoo.com.cn

Citing This Article：

LU Jun, DONG Hui-Yue, LIN Ling-Jing, WANG Qing-Hua, HUANG Liang-Hu, TAN Jian-Ming. [Palmitate induces apoptosis and endoplasmic reticulum stress in human umbilical cord-derived mesenchymal stem cells.] [Article in Chinese]. Acta Physiol Sin 2013; 65 (6): 600-606 (in Chinese with English abstract).