ISSN 0371-0874, CN 31-1352/Q

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生理学报 2006; 58(5): 415-420

强制运动促进成年大鼠海马齿状回神经发生并提高学习能力

徐卫平, 单立冬, 龚珊, 陈琳, 张跃进, 印其章, HISAMITSU Tadashi, 蒋星红, 郭试瑜

苏州大学医学院神经生物学教研室，苏州 215123；昭和大学医学部第一生理学教研室，东京 142，日本

摘要

为了探讨强制运动对成年大鼠海马齿状回(dentate gyrus, DG)神经发生的影响，强制大鼠在马达驱动的转轮中跑步，用5- 溴-2- 脱氧尿苷(5-bromo-2-deoxyuridine, BrdU)标记增殖细胞，巢蛋白(neuroepthelial stem cell protein, nestin)标记神经干细胞/前体细胞，然后用免疫细胞化学技术检测大鼠DG 中BrdU 及nestin 阳性细胞。为了解强制运动后DG 增殖细胞的功能意义，采用Y- 迷宫检测大鼠的学习能力。结果表明，强制运动组DG 中BrdU 及nestin 阳性细胞数均明显多于对照组(P<0.05); 强制运动对DG 神经发生的效应有强度依赖性。Y- 迷宫检测结果显示，强制运动能明显改善大鼠的学习能力。结果提示，在转轮中进行强制跑步能促进成年大鼠DG 的神经发生，并改善学习能力。

关键词： 海马; 神经发生; 齿状; 跑步; 5 -溴-2-脱氧尿苷; 免疫组织化学; 迷宫学习; 大鼠

Forced running enhances neurogenesis in the hippocampal dentate gyrus of adult rats and improves learning ability

XU Wei-Ping, SHAN Li-Dong, GONG Shan, CHEN Lin, ZHANG Yue-Jin, YIN QI-ZHANG, HISAMITSU Tadashi, JIANG Xing-Hong, GUO Shi-Yu

Department of Neurobiology, School of Medicine, Soochow University, Suzhou 215123, China；Department of Physiology, School of Medicine, Showa University, Tokyo 142, Japan

Abstract

To investigate the effect of forced running in motor-driven wheel on neurogenesis in the hippocampal dentate gyrus (DG)of adult rats, 5-bromo-2-deoxyuridine (BrdU), a thymidine analog was applied to mark cell proliferation. Neuroepthelial stem cellprotein (nestin) expression was used to identify neural stem/precursor cells. The BrdU- and nestin-positive cells were examined byimmunohistochemical technique. The ability of learning was evaluated by Y-maze test to explore the functional role of the newborn cellsin the DG after forced running. It was found that the number of BrdU- and nestin-positive cells in the DG in running groups wassignificantly increased compared to that in the control group (P<0.05). The effect of forced running on neurogenesis was intensitydependent.In addition, an improvement of learning ability in Y-maze test was observed after forced running. These findings suggest thatforced running in motor-driven wheel could enhance neurogenesis in the hippocampal DG of adult rats and improve learning ability.

Key words: Hippocampus; neurogenesis; dentate gyrus; running; 5-bromo-2-deoxyuridine; immunohistochemistry; maze learning; rats

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通讯作者：郭试瑜　　E-mail: szyinqz@pub.sz.jsinfo.net

引用本文：

徐卫平, 单立冬, 龚珊, 陈琳, 张跃进, 印其章, HISAMITSU Tadashi, 蒋星红, 郭试瑜. 强制运动促进成年大鼠海马齿状回神经发生并提高学习能力[J]. 生理学报 2006; 58 (5): 415-420.

XU Wei-Ping, SHAN Li-Dong, GONG Shan, CHEN Lin, ZHANG Yue-Jin, YIN QI-ZHANG, HISAMITSU Tadashi, JIANG Xing-Hong, GUO Shi-Yu. Forced running enhances neurogenesis in the hippocampal dentate gyrus of adult rats and improves learning ability. Acta Physiol Sin 2006; 58 (5): 415-420 (in Chinese with English abstract).

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